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human estrogen e elisa kit  (Cusabio)


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    Cusabio human estrogen e elisa kit
    Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) <t>ELISA</t> was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
    Human Estrogen E Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+estrogen+e+elisa+kit/Human+estrogen%2CE+ELISA+Kit/pm40380255-87-2-7
    Average 93 stars, based on 9 article reviews
    human estrogen e elisa kit - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis."

    Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis.

    Journal: Journal of ovarian research

    doi: 10.1186/s13048-025-01683-6

    Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) ELISA was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) ELISA was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: CCK-8 Assay, Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

    Fig. 8 NEAT1 regulates steroid hormone biosynthesis and the MAPK signaling pathway in KGN cells via the miR-204-5p/ESR1 axis. (a-b) RT-qPCR and western blotting were performed to evaluate the effect of NEAT1 on ESR1 mRNA and protein expression levels, respectively. (c) RT-qPCR was performed to analyze the expression of key genes involved in steroid hormone synthesis. (d) ELISA was performed to determine E2 levels. (e) western blotting was performed to determine the levels of proteins related to steroid hormone synthesis and MAPK signaling pathway. (f-g) RT-qPCR and western blotting were used to assess the expression levels of steroid synthase and key molecules of the MAPK pathway in human GCs, respectively. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: Fig. 8 NEAT1 regulates steroid hormone biosynthesis and the MAPK signaling pathway in KGN cells via the miR-204-5p/ESR1 axis. (a-b) RT-qPCR and western blotting were performed to evaluate the effect of NEAT1 on ESR1 mRNA and protein expression levels, respectively. (c) RT-qPCR was performed to analyze the expression of key genes involved in steroid hormone synthesis. (d) ELISA was performed to determine E2 levels. (e) western blotting was performed to determine the levels of proteins related to steroid hormone synthesis and MAPK signaling pathway. (f-g) RT-qPCR and western blotting were used to assess the expression levels of steroid synthase and key molecules of the MAPK pathway in human GCs, respectively. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis.
    Article Snippet: .. Subsequently, a Human Estrogen E ELISA Kit (Cusabio, Wuhan, China) was used according to the manufacturer’s instructions, and the optical density was measured at 450 nm using an enzyme marker. ..

    Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis
    Article Snippet: .. Subsequently, a Human Estrogen E ELISA Kit (Cusabio, Wuhan, China) was used according to the manufacturer’s instructions, and the optical density was measured at 450 nm using an enzyme marker. ..

    Marker:

    Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis.
    Article Snippet: .. Subsequently, a Human Estrogen E ELISA Kit (Cusabio, Wuhan, China) was used according to the manufacturer’s instructions, and the optical density was measured at 450 nm using an enzyme marker. ..

    Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis
    Article Snippet: .. Subsequently, a Human Estrogen E ELISA Kit (Cusabio, Wuhan, China) was used according to the manufacturer’s instructions, and the optical density was measured at 450 nm using an enzyme marker. ..



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    Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) <t>ELISA</t> was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
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    Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) <t>ELISA</t> was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
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    Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) <t>ELISA</t> was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
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    Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) <t>ELISA</t> was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
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    Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) <t>ELISA</t> was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
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    Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) ELISA was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Journal of ovarian research

    Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis.

    doi: 10.1186/s13048-025-01683-6

    Figure Lengend Snippet: Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) ELISA was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: Subsequently, a Human Estrogen E ELISA Kit (Cusabio, Wuhan, China) was used according to the manufacturer’s instructions, and the optical density was measured at 450 nm using an enzyme marker.

    Techniques: CCK-8 Assay, Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

    Fig. 8 NEAT1 regulates steroid hormone biosynthesis and the MAPK signaling pathway in KGN cells via the miR-204-5p/ESR1 axis. (a-b) RT-qPCR and western blotting were performed to evaluate the effect of NEAT1 on ESR1 mRNA and protein expression levels, respectively. (c) RT-qPCR was performed to analyze the expression of key genes involved in steroid hormone synthesis. (d) ELISA was performed to determine E2 levels. (e) western blotting was performed to determine the levels of proteins related to steroid hormone synthesis and MAPK signaling pathway. (f-g) RT-qPCR and western blotting were used to assess the expression levels of steroid synthase and key molecules of the MAPK pathway in human GCs, respectively. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Journal of ovarian research

    Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis.

    doi: 10.1186/s13048-025-01683-6

    Figure Lengend Snippet: Fig. 8 NEAT1 regulates steroid hormone biosynthesis and the MAPK signaling pathway in KGN cells via the miR-204-5p/ESR1 axis. (a-b) RT-qPCR and western blotting were performed to evaluate the effect of NEAT1 on ESR1 mRNA and protein expression levels, respectively. (c) RT-qPCR was performed to analyze the expression of key genes involved in steroid hormone synthesis. (d) ELISA was performed to determine E2 levels. (e) western blotting was performed to determine the levels of proteins related to steroid hormone synthesis and MAPK signaling pathway. (f-g) RT-qPCR and western blotting were used to assess the expression levels of steroid synthase and key molecules of the MAPK pathway in human GCs, respectively. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: Subsequently, a Human Estrogen E ELISA Kit (Cusabio, Wuhan, China) was used according to the manufacturer’s instructions, and the optical density was measured at 450 nm using an enzyme marker.

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay